Journal of Advances in Molecular Biology

Download PDF (9118 KB) PP. 27 - 41 Pub. Date: December 15, 2018

DOI: 10.22606/jamb.2018.24001

• Liliya Vitanova^*
  Department of Physiology, Medical University of Sofia, Bulgaria

The aim of the present study was to elucidate the putative interactions between the serotoninergic and the GABAergic systems in the lower vertebrate retinas. Experiments were performed on 12-14 µm frozen frog and turtle retinal sections, processed with more than 10 different antibodies directed against serotonin, GABA and their membrane receptors by means of the indirect immunocytochemical method. The results showed that some of the GABAergic retinal neurons possess the serotonin receptors 5-HT2A and 5-HT3A on their cell membrane, and vice versa: certain serotoninergic neurons possess the ionotropic GABAA and GABAC receptors. A simple neuronal chart is proposed to explain how the activation of serotonin receptors on a subgroup of GABAergic amacrine cells can decrease the GABAC receptor mediated inhibition on the retinal bipolar cells, the main generators of ERG. As the retina is a natural biological model of CNS, the relationships between both neurotransmitter systems described here may elucidate their participation in certain brain functions and to explain the possible mechanisms of action of some antidepressant drugs.

Retina, neurotransmitters’ interactions, serotonin, GABAA and GABAC receptors, 5-HT2A and 5-HT3A receptors.

[1] Ehinger B (1983). Connexions between retinal neurons with identified neurotransmitters. Vision Res; 23(11):1281-91.

[2] Vígh J, Bánv?lgyi T, Wilhelm M. (2000). Amacrine cells of the anuran retina: morphology, chemical neuroanatomy, and physiology. Microsc Res Tech 2000; 50(5):373-83.

[3] Zhu B, Gábriel R, Straznicky C(1992). Serotonin synthesis and accummulation by neurons of the anuran retina. Vis Neurosci 1992; 9(3-4):377-88.

[4] Ghai K, Zelinka C, Fischer AJ (2009). Serotonin released from amacrine neurons is scavenged and degraded in bipolar neurons in the retina. Neurochem. 111(1):1-14. doi: 10.1111/j.1471-4159.2009.06270.x.

[5] Schütte M, Weiler R. Morphometric analysis of serotoninergic bipolar cells in the retina and its implications for retinal image processing. J Comp Neurol 1987; 260(4):619-26.

[6] Schütte M. Serotonergic and serotonin-synthesizing cells of the Xenopus retina. Int J Neurosci 1994; 8(1-2), 67-73.

[7] Vitanova L, Kupenova P, Zhekova D (2017a). Tryptophan hydroxylase and serotonin receptors 5-HT1A, 5-HT2A, 5-HT3A, 5-HT4, 5-HT 5A, 5-HT 6 and 5-HT 7 in frog and turtle retina: an immunofluorescence study. J Adv Mol Biol., 1(1), 33-72.

[8] Cornide-Petronio ME, Anadón R, Rodicio MC, Barreiro-Iglesias A (2013). The sea lamprey tryptophan hydroxylase: new insight into the evolution of the serotonergic system of vertebrates. Brain Struct Funct., 218(2): 587-93.

[9] Cornide-Petronio ME, Anadón R, Barreiro-Iglesias A, Rodicio MC (2015). Tryptophan hydroxylase and serotonin receptor 1A expression in the retina of the sea lamprey. Exp Eye Res, 135:81-7.

[10] Nichols DE, Nichols CD. (2008) Serotonin receptors. Chem. Rev, 108(5), 1614 – 164. doi: 10.1021/cr078224o. Epub 2008 May 14. Review.

[11] Olsen RW, Sieghart W. (2009). GABA A receptors subtypes provide diversity of function and pharmacology, Neuropharmacol 56, 141-8.

[12] Vitanova L, Kupenova P, Haverkamp S, Popova E, Mitova L, W?ssle H (2001). Immunocytochemical and electrophysiological characterization of GABA receptors in the frog and turtle retina. Vision Res. 41(6): 691-704.

[13] Ochoa-de la Paz LD, Estrada-Mondragón A, Limón A, Miledi R, Martínez-Torres A (2012). Dopamine and serotonin modulate human GABAρ1 receptors expressed in Xenopus laevis oocytes. ACS Chem Neurosci, 3(2):96-104.

[14] Hurd LB 2nd, Eldred WD. (1993). Synaptic microcircuitry of bipolar and amacrine cells with serotonin-like immunoreactivity in the retina of the turtle, Pseudemys scripta elegans. Vis Neurosci;10(3):455-71.

[15] Gábriel R, Zhu BS, Straznicky C (1993). Synaptic contacts of serotonin-like immunoreactive and 5,7-dihydroxytryptamine-accumulating neurons in the anuran retina. Neuroscience.54(4):1103-14.

[16] Gastinger MJ, Bordt AS, Bernal MP, Marshak DW (2005). Serotonergic retinopetal axons in the monkey retina. Curr Eye Res, 30(12):1089-95.

[17] Lima L, Urbina M (1998). Serotonergic projections to the retina of rat and goldfish; Neurochem, 32(2):133-4.

[18] Lukasiewicz PD, Eggers ED, Sagdullaev BT, McCall MA. (2004). GABAC receptor-mediated inhibition in the retina; 44(28):3289-96. https://www.ncbi.nlm.gov/pubmed/155359996.

[19] Vitanova L, Kupenova P, Popova E. (2017b). Immunocytochemical study on the possible interactions between serotonin and GABA C (GABA A rho) receptors in frog retina. IUPS 38th World Congress, Abst. No576. (https://icongresso.itarget.com.br/useradm/anais/?clt=ips.2&lng=I).

[20] Popova E, Kupenova P, Vitanova L. (2017). Interaction between the serotoninergic and GABAergic systems in the frog retina as revealed by the electroretinogram. IUPS 38th World Congress, Abst..No 568 (https://icongresso.itarget.com.br/useradm/anais/?clt=ips.2&lng=I).

[21] Popova E., Kupenova P. (2017) Interaction between the serotoninergic and GABAergic system in frog retina, as reveales by electroretinogram. Acta Neurobiol.Exp, 77: 351 – 36.

[22] Fletcher EL, Waessle K (1999). Indoleamine-accumulating amacrine cells are presynaptic to rod bipolar cells through GABA(C) receptors. J Comp Neurol. 1999; 413(1):155-67.

[23] Eggers ED, Lukasiewicz PD (2010). Interneuron circuits tune inhibition in retinal bipolar cells. J Neurophysiol.;103(1):25-37.

[24] Eggers ED, Lukasiewicz PD (2011). Multiple pathways of inhibition shape bipolar cell responses in the retina. Vis Neurosci.; 28(1):95-108.

[25] Travis AM, Heflin SJ, Hirano AA, Brecha NC and Arshavsky VY (2018). Dopamine-dependent sensitization of rod bipolar cells by GABA is conveyed through wide-field amacrine cells. J. Neurosci, 38(3): 723-732.

[26] Bagdy E, Kiraly I, Harsing LG Jr. (2000). Reciprocal innervation between serotonergic and GABAergic neurons in raphe nuclei of the rat. Neurochem Res; 25(11):1465-73.

[27] Feng J, Cai X, Zhao J, Yan Z. (2001). Serotonin receptors modulate GABA(A) receptor channels through activation of anchored protein kinase C in prefrontal cortical neurons, 21(17):6502-11.